Kdm4b^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kdm4b^{tm1c(EUCOMM)Wtsi}
• Name: lysine (K)-specific demethylase 4B; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5792925
• Synonyms: Jmjd2b^f
• Gene: Kdm4b Location: Chr17:56633062-56709870 bp, + strand Genetic Position: Chr17, 29.27 cM
• Alliance: Kdm4b^{tm1c(EUCOMM)Wtsi} page
• IMPC: Kdm4b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234086
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 56662423 of Chromosome 17 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56663316. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, deleting the lacZ sequence and neomycin selection cassette. The critical exon 5 remains flanked by loxP sites. (J:234086)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm4b Mutation: 55 strains or lines available

References

• Original: J:234086 Pedersen MT, et al., Continual removal of H3K9 promoter methylation by Jmjd2 demethylases is vital for ESC self-renewal and early development. EMBO J. 2016 Jul 15;35(14):1550-64
• All: 5 reference(s)