Kdm4a^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kdm4a^{tm1c(EUCOMM)Wtsi}
• Name: lysine (K)-specific demethylase 4A; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5792924
• Synonyms: Jmjd2a^f
• Gene: Kdm4a Location: Chr4:117994154-118037216 bp, - strand Genetic Position: Chr4, 54.31 cM
• Alliance: Kdm4a^{tm1c(EUCOMM)Wtsi} page
• IMPC: Kdm4a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234086
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 118032444 of Chromosome 4 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 118033390. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, deleting the lacZ sequence and neomycin selection cassette. LoxP sites flank exon 3. (J:234086)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm4a Mutation: 45 strains or lines available

References

• Original: J:234086 Pedersen MT, et al., Continual removal of H3K9 promoter methylation by Jmjd2 demethylases is vital for ESC self-renewal and early development. EMBO J. 2016 Jul 15;35(14):1550-64
• All: 3 reference(s)