Sparc^{tm2.1(SPARC)Amde}
Targeted Allele Detail

Summary

• Symbol: Sparc^{tm2.1(SPARC)Amde}
• Name: secreted acidic cysteine rich glycoprotein; targeted mutation 2.1, Anne M Delany
• MGI ID: MGI:5790983
• Synonyms: ON^B
• Gene: Sparc Location: Chr11:55284985-55310906 bp, - strand Genetic Position: Chr11, 33.04 cM, cytoband B1
• Alliance: Sparc^{tm2.1(SPARC)Amde} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233364
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Sparc^{tm2.1(SPARC)Amde} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SPARC (6678)

  Sparc

  (MGI:98373)

  3' human UTR replaced mouse 3' UTR

• Mutation details: Sequence from the human ortholog (consisting of the 1 kb 3' UTR from haplotype B which has the C allele of SNP rs1054204, the consensus polyA site and a G-T rich region), preceded by a loxP site flanked neomycin resistance gene cassette, was flanked by 8-10 kb long mouse homology arms. This construct was targeted to the mouse gene and used to replace the endogenous 3' UTR in exon 10 with the human 3' UTR. Subsequent cre-mediated recombination excised the neo cassette. (J:233364)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sparc Mutation: 76 strains or lines available

References

• Original: J:233364 Dole NS, et al., A single nucleotide polymorphism in osteonectin 3' untranslated region regulates bone volume and is targeted by miR-433. J Bone Miner Res. 2015 Apr;30(4):723-32
• All: 2 reference(s)