Gt(ROSA)26Sor^{tm9.1(CAG-mCherry,-CHRM4*)Dym}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm9.1(CAG-mCherry,-CHRM4*)Dym}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 9.1, Susan Dymecki
• MGI ID: MGI:5790684
• Synonyms: RC::PDi
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm9.1(CAG-mCherry,-CHRM4*)Dym} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234693
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced into the locus a CAG promoter, FRT-flanked STOP cassette, loxP site, mCherry reporter gene, STOP cassette, loxP site and hM4Di sequence is a Gi-coupled human M4 muscarinic DREADD (designer receptor exclusively activated by designer drug). To create the hM4Di sequence, the wildtype human muscarinic 4 receptor (CHRM4) sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y113C<3.33>/A203G<5.46>) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). Flp-mediated recombination removed the first STOP cassette allowing for expression of the mCherry reporter gene. Cre-mediated recombination is required to achieve expression of the hM4Di sequence. (J:234693)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:234693 Ray RS, et al., Impaired respiratory and body temperature control upon acute serotonergic neuron inhibition. Science. 2011 Jul 29;333(6042):637-42
• All: 8 reference(s)