Gt(ROSA)26Sortm9(CAG-mCherry,-CHRM4*)Dym
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm9(CAG-mCherry,-CHRM4*)Dym |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 9, Susan Dymecki |
MGI ID: |
MGI:5790683 |
Synonyms: |
RC::FPDi |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm9(CAG-mCherry,-CHRM4*)Dym page
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Germline Transmission: |
Earliest citation of germline transmission:
J:234693
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Parent Cell Line: |
TC1/TC-1 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, Reporter) |
Mutation: |
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Insertion
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Mutation details: The targeting construct introduced into the locus a CAG promoter, FRT-flanked STOP cassette, loxP site, mCherry reporter gene, STOP cassette, loxP site and hM4Di sequence is a Gi-coupled human M4 muscarinic DREADD (designer receptor exclusively activated by designer drug). To create the hM4Di sequence, the wildtype human muscarinic 4 receptor (CHRM4) sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y113C<3.33>/A203G<5.46>) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). Flp-mediated recombination is required to remove the first STOP cassette and allow for expression of the mCherry reporter gene. Cre-mediated recombination is required to achieve expression of the hM4Di sequence.
(J:234693)
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Original: |
J:234693 Ray RS, et al., Impaired respiratory and body temperature control upon acute serotonergic neuron inhibition. Science. 2011 Jul 29;333(6042):637-42 |
All: |
9 reference(s) |
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