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Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm9(CAG-mCherry,-CHRM4*)Dym
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 9, Susan Dymecki
MGI ID: MGI:5790683
Synonyms: RC::FPDi
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Germline Transmission:  Earliest citation of germline transmission: J:234693
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
Mutation detailsThe targeting construct introduced into the locus a CAG promoter, FRT-flanked STOP cassette, loxP site, mCherry reporter gene, STOP cassette, loxP site and hM4Di sequence is a Gi-coupled human M4 muscarinic DREADD (designer receptor exclusively activated by designer drug). To create the hM4Di sequence, the wildtype human muscarinic 4 receptor (CHRM4) sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y113C<3.33>/A203G<5.46>) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). Flp-mediated recombination is required to remove the first STOP cassette and allow for expression of the mCherry reporter gene. Cre-mediated recombination is required to achieve expression of the hM4Di sequence. (J:234693)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  572 strains or lines available
Original:  J:234693 Ray RS, et al., Impaired respiratory and body temperature control upon acute serotonergic neuron inhibition. Science. 2011 Jul 29;333(6042):637-42
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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