Tg(Gad2-tdTomato)DJhi
Transgene Detail

Summary

• Symbol: Tg(Gad2-tdTomato)DJhi
• Name: transgene insertion D, Johannes Hirrlinger
• MGI ID: MGI:5790577
• Synonyms: TgN(GAD65-tdTomato)
• Transgene: Tg(Gad2-tdTomato)DJhi Location: unknown
• Alliance: Tg(Gad2-tdTomato)DJhi page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A sequence was engineered comprising the tdTomato red fluorescent protein open reading frame followed by the SV40 polyadenylation sequence and an FRT site-flanked neomycin resistance cassette. This was inserted into the BAC RP23-407K8 immediately downstream of the translation-initiating ATG in exon 1 of the mouse glutamic acid decarboxylase 2 gene (Gad2; also called Gad65); the BAC contains all 16 exons, 72 kb of 5'- and 64 kb of 3'-flanking DNA. The neo cassette was removed by flp-mediated recombination. Of 11 founder mouse lines that had integrated the transgene and that transmitted it through the germline, only one exhibited the expected expression pattern upon fluorescence imaging of brain sections. (J:233808)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:233808 Besser S, et al., A Transgenic Mouse Line Expressing the Red Fluorescent Protein tdTomato in GABAergic Neurons. PLoS One. 2015;10(6):e0129934
• All: 6 reference(s)