Hotair^tm1.1Hyc
Targeted Allele Detail

Summary

• Symbol: Hotair^tm1.1Hyc
• Name: HOX transcript antisense RNA (non-protein coding); targeted mutation 1.1, Howard Chang
• MGI ID: MGI:5789897
• Synonyms: Hotair KO, Hoxc^tm1.1Hyc
• Gene: Hotair Location: Chr15:102852494-102856165 bp, - strand Genetic Position: Chr15, 58.01 cM
• Alliance: Hotair^tm1.1Hyc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:203783
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The targeting vector was designed to insert a loxP site 5' of exon 1 and a loxP-flanked neomycin cassette in intron 2. Exons 1 and 2 comprise the full length gene. Cre-mediated recombination removed the floxed region. Quantitative RT-PCR analysis did not detect mRNA in homozygous mutant mice. (J:203783)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hotair Mutation: 2 strains or lines available

References

• Original: J:203783 Li L, et al., Targeted disruption of Hotair leads to homeotic transformation and gene derepression. Cell Rep. 2013 Oct 17;5(1):3-12
• All: 4 reference(s)