Gdf5^{tm1.1(cre/ERT2)Eze}
Targeted Allele Detail

Summary

• Symbol: Gdf5^{tm1.1(cre/ERT2)Eze}
• Name: growth differentiation factor 5; targeted mutation 1.1, Elazar Zelzer
• MGI ID: MGI:5789619
• Synonyms: Gdf5-CreER, Gdf5-CreER^T2
• Gene: Gdf5 Location: Chr2:155782943-155787204 bp, - strand Genetic Position: Chr2, 77.26 cM
• Alliance: Gdf5^{tm1.1(cre/ERT2)Eze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234264
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intergenic deletion
• Gdf5^{tm1.1(cre/ERT2)Eze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gdf5 (MGI:95688)

• Mutation details: A targeting vector was designed to insert a Cre/ERT2 fusion gene, an SV40 polyA signal, and an frt-flanked neo-polyA cassette into the translation start site of Gdf5 gene. This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6J-congenic ROSA26::FLPe knock-in females to remove the neo selection cassette and to originate the colony. (J:234264)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gdf5 (mouse) Summary of all recombinase alleles driven by Gdf5. Alliance mouse Gdf5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gdf5 Mutation: 27 strains or lines available

References

• Original: J:234264 Shwartz Y, et al., Joint Development Involves a Continuous Influx of Gdf5-Positive Cells. Cell Rep. 2016 Jun 21;15(12):2577-87
• All: 4 reference(s)