Cercamem1(IMPC)J
Endonuclease-mediated Allele Detail
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Symbol: |
Cercamem1(IMPC)J |
Name: |
cerebral endothelial cell adhesion molecule; endonuclease-mediated mutation 1, Jackson |
MGI ID: |
MGI:5788503 |
Synonyms: |
Cercamem1J |
Gene: |
Cercam Location: Chr2:29759176-29772852 bp, + strand Genetic Position: Chr2, 20.76 cM, cytoband B
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Alliance: |
Cercamem1(IMPC)J page
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IMPC: |
Cercam gene page |
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Strain of Origin: |
C57BL/6NJ
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Project Collection: |
IMPC
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Allele Type: |
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Endonuclease-mediated (Null/knockout) |
Mutation: |
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Intragenic deletion
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Mutation details: This allele from project Cercam-7869J-F7768 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences AATTCCCCCTCAGTGAACTC, AGGGAATCCTGTCTCAGACT, CTAGGCAGTAAATTTAGGAA and ACTAGCCCTTCTCCTCCACT, which resulted in a 674 bp deletion beginning at Chromosome 2 positive strand position 29,872,506 bp, TGAACTCTGGTCCCGAGTCT, and ending after CCTAAATTTACTGCCTAGTG at 29,873,179 bp (GRCm38/mm10). This mutation deletes exons 4 and 5 and 334 bp of flanking intronic sequence including the splice acceptors and donors. In addition there is a 13 bp insertion (agggaatttctca) at the site of the deletion that will not alter the results of the deletion, which is predicted to cause a change of amino acid sequence after residue 139 and early truncation 14 amino acids later.
(J:188991)
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Inheritance: |
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Not Specified |
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Original: |
J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012; |
All: |
2 reference(s) |
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