Cfl2^tm1.2Wit
Targeted Allele Detail

Summary

• Symbol: Cfl2^tm1.2Wit
• Name: cofilin 2, muscle; targeted mutation 1.2, Walter Witke
• MGI ID: MGI:5788501
• Gene: Cfl2 Location: Chr12:54905594-54909662 bp, - strand Genetic Position: Chr12, 23.75 cM, cytoband C1
• Alliance: Cfl2^tm1.2Wit page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233564
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 2 through 4. Western blot analysis confirmed the absence of protein expression in skeletal muscle. (J:233564)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cfl2 Mutation: 17 strains or lines available

References

• Original: J:233564 Gurniak CB, et al., Severe protein aggregate myopathy in a knockout mouse model points to an essential role of cofilin2 in sarcomeric actin exchange and muscle maintenance. Eur J Cell Biol. 2014 May-Jun;93(5-6):252-66
• All: 1 reference(s)