Spi1^tm1.1Nerl
Targeted Allele Detail

Summary

• Symbol: Spi1^tm1.1Nerl
• Name: Spi-1 proto-oncogene; targeted mutation 1.1, Claus Nerlov
• MGI ID: MGI:5787793
• Synonyms: PU.1^eYFP, PU.1^YFP
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm1.1Nerl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:112660
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: Using RecE-RecT recombination, the YFP coding sequence was inserted immediately upstream of the stop codon and a loxP site flanked neomycin resistance gene cassette was inserted 3' of the locus. Subsequent cre-mediated recombination deleted the neo cassette. This creates a gene coding for a fusion protein between the target and YFP. (J:112660)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 27 strains or lines available

References

• Original: J:112660 Kirstetter P, et al., Activation of the canonical Wnt pathway leads to loss of hematopoietic stem cell repopulation and multilineage differentiation block. Nat Immunol. 2006 Oct;7(10):1048-56
• All: 5 reference(s)