Ercc5^tm1.2Jhjh
Targeted Allele Detail

Summary

• Symbol: Ercc5^tm1.2Jhjh
• Name: excision repair cross-complementing rodent repair deficiency, complementation group 5; targeted mutation 1.2, Jan H J Hoeijmakers
• MGI ID: MGI:5787631
• Synonyms: Xpg^-
• Gene: Ercc5 Location: Chr1:44186904-44220420 bp, + strand Genetic Position: Chr1, 23.55 cM, cytoband B
• Alliance: Ercc5^tm1.2Jhjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232544
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 3. Western blot analysis confirmed the absence of protein expression in dermal fibroblasts. (J:232544)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ercc5 Mutation: 53 strains or lines available

References

• Original: J:232544 Barnhoorn S, et al., Cell-autonomous progeroid changes in conditional mouse models for repair endonuclease XPG deficiency. PLoS Genet. 2014 Oct;10(10):e1004686
• All: 5 reference(s)