Rorb^{tm2.1(tTA2)Hze}
Targeted Allele Detail

Summary

• Symbol: Rorb^{tm2.1(tTA2)Hze}
• Name: RAR-related orphan receptor beta; targeted mutation 2.1, Hongkui Zeng
• MGI ID: MGI:5784556
• Synonyms: Rorb-2A-tTA2-D
• Gene: Rorb Location: Chr19:18907969-19088560 bp, - strand Genetic Position: Chr19, 13.28 cM
• Alliance: Rorb^{tm2.1(tTA2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Transactivator)
• Mutation: Insertion
• Mutation details: The targeting vector contains, from 5' to 3',a partial Rorb intron 9 containing an FRT3 site, a partial Ndnf exon 10 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Rorb coding sequence,a synthetic modified tetracycline-regulated transactivator (tTA2) gene, polyA signal, an AttB site, a PGK-Neo-polyA cassette, an FRT5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::FRT5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rorb Mutation: 38 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)