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Transgene Detail
Symbol: Tg(tetO/Prnp-GLRA1*,-EYFP)9492Jjan
Name: transgene insertion 9492, Joanna L Jankowsky
MGI ID: MGI:5779807
Synonyms: TRE-GlyCl 9492
Transgene: Tg(tetO/Prnp-GLRA1*,-EYFP)9492Jjan  Location: unknown  
Strain of Origin:  (C57BL/6J x C3H/HeJ)F2
Transgene Type:    Transgenic (Humanized sequence, Inducible, Reporter)
Mutation:    Insertion
Mutation detailsThe transgene has a Tet-responsive element 3G promoter and the mouse prion protein non-coding exons 1-2 upstream of a cassette containing an enhanced yellow fluorescent reporter (EYFP) flanked on either side by a human glycine receptor alpha1 (GLRA1) cDNA sequence harboring the point mutations F207A (to eliminate endogenous ligand [glycine] sensitivity) and A288G (to increase ivermectin sensitivity almost 100-fold), followed by the mouse prion protein non-coding exon 3 and the woodchuck hepatitis post-transcriptional regulatory element (WPRE). To achieve tricistronic expression of the point mutations and EYFP, they are separated by T2A peptide sequences (from Thosea asigna virus). (J:232624)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:232624 Zhao R, et al., Impaired Recall of Positional Memory following Chemogenetic Disruption of Place Field Stability. Cell Rep. 2016 Jul 19;16(3):793-804
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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