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Transgene Detail
Symbol: Tg(tetO/Prnp-GLRA1*,-EYFP)9487Jjan
Name: transgene insertion 9487, Joanna L Jankowsky
MGI ID: MGI:5779695
Synonyms: TRE-GlyCl 9487
Transgene: Tg(tetO/Prnp-GLRA1*,-EYFP)9487Jjan  Location: unknown  
Strain of Origin:  (C57BL/6J x C3H/HeJ)F2
Transgene Type:    Transgenic (Humanized sequence, Inducible, Reporter)
Mutation:    Insertion
Mutation detailsThe transgene has a Tet-responsive element 3G promoter and the mouse prion protein non-coding exons 1-2 upstream of a cassette containing an enhanced yellow fluorescent reporter (EYFP) flanked on either side by a human glycine receptor alpha1 (GLRA1) cDNA sequence harboring the point mutations F207A (to eliminate endogenous ligand [glycine] sensitivity) and A288G (to increase ivermectin sensitivity almost 100-fold), followed by the mouse prion protein non-coding exon 3 and the woodchuck hepatitis post-transcriptional regulatory element (WPRE). To achieve tricistronic expression of the point mutations and EYFP, they are separated by T2A peptide sequences (from Thosea asigna virus). Lines 9487 and 9511 display the weakest overall signal. (J:232624)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:232624 Zhao R, et al., Impaired Recall of Positional Memory following Chemogenetic Disruption of Place Field Stability. Cell Rep. 2016 Jul 19;16(3):793-804
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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