Tg(Prnp/PRNP*ARR)FM18Pcg
Transgene Detail

Summary

• Symbol: Tg(Prnp/PRNP*ARR)FM18Pcg
• Name: trangene insertion FM18, Peter Griffiths
• MGI ID: MGI:5779521
• Synonyms: Tg(OvPrP)FM18
• Transgene: Tg(Prnp/PRNP*ARR)FM18Pcg Location: unknown
• Alliance: Tg(Prnp/PRNP*ARR)FM18Pcg page

Transgene origin

• Strain of Origin: FVB/NIco

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp/PRNP*ARR)FM18Pcg expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)
  sheep	PRNP (493887)	Mouse/sheep chimeric protein

• Mutation details: The 12-kb transgene, derived from the pPrHG "half genomic" vector, contains the promoter and first exon of the mouse prion protein gene (Prnp), mouse exon 2 joined to a chimeric mouse/sheep exon 3, and the 3'-untranslated region (UTR) of the mouse gene. The chimeric exon contains nucleotides (nt) 1-117 of the mouse Prnp coding sequence, encoding amino acids (aa) 1-39, and nt 118-762 of the sheep PRNP coding sequence encoding aa 40-253 of the ARR prion protein variant, which has alanine, arginine and arginine, respectively, at ovine polymorphic amino acid positions 136, 154 and 171 (A136R154R171). Mice bearing this transgene express mouse/ovine Prnp/PRNP^ARR from the mouse Prnp promoter in brain and other tissues. (J:32214, J:82809, J:232471)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:232471 Veterinary Laboratories Agency, Preparation of more sensitive bioassay models for the improved detection, differentiation and diagnosis of the BSE agent. SID 5 Research Project Final Report, DEFRA Project Code SE1753. 2009
• All: 3 reference(s)