Igh^{tm3.1(VRC01)Nemz}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm3.1(VRC01)Nemz}
• Name: immunoglobulin heavy chain complex; targeted mutation 3.1, David Nemazee
• MGI ID: MGI:5779356
• Synonyms: VRC01 gH
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225518
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette replaced the DQ52-JH cluster in the Igh locus with the HIV broadly neutralizing antibody precursor VRC01 gH VDJ exon. The upstream promoter, leader and intron elements were from VHJ558.85.191. Cre-mediated recombination removed the neomycin resistance cassette. (J:225518)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:225518 Jardine JG, et al., HIV-1 VACCINES. Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen. Science. 2015 Jul 10;349(6244):156-61
• All: 6 reference(s)