Mogat2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mogat2^{tm1c(KOMP)Wtsi}
• Name: monoacylglycerol O-acyltransferase 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5779196
• Synonyms: Mogat2^f
• Gene: Mogat2 Location: Chr7:98868291-98887818 bp, - strand Genetic Position: Chr7, 54.03 cM
• Alliance: Mogat2^{tm1c(KOMP)Wtsi} page
• IMPC: Mogat2 gene page

Mutation origin

• Mutant Cell Line: EPD0093_1_A12
• Germline Transmission: Earliest citation of germline transmission: J:232070
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98881428 of Chromosome 7 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 98882313. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:232070)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mogat2 Mutation: 21 strains or lines available

Notes

Cell line EPD0093_1_C12 was successfully used to make chimeric mice. Germline transmission was accomplished. J:232070

References

• Original: J:232070 Nelson DW, et al., Intestine-specific deletion of acyl-CoA:monoacylglycerol acyltransferase (MGAT) 2 protects mice from diet-induced obesity and glucose intolerance. J Biol Chem. 2014 Jun 20;289(25):17338-49
• All: 1 reference(s)