Tg(Prnp/PRNP*AHQ)EM16Pcg
Transgene Detail

Summary

• Symbol: Tg(Prnp/PRNP*AHQ)EM16Pcg
• Name: transgene insertion EM16, Peter Griffiths
• MGI ID: MGI:5776247
• Synonyms: EM16, Tg(Mu/OvPrP^AHQ)EM16, Tg(OvPrP)EM16
• Transgene: Tg(Prnp/PRNP*AHQ)EM16Pcg Location: unknown
• Alliance: Tg(Prnp/PRNP*AHQ)EM16Pcg page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp/PRNP*AHQ)EM16Pcg expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  sheep	PRNP (493887)	Mouse/sheep chimeric protein

• Mutation details: The 12-kb transgene, derived from the pPrHG "half genomic" vector, contains the promoter and first exon of the mouse prion protein gene (Prnp), mouse exon 2 joined to a chimeric mouse/sheep exon 3, and the 3'-untranslated region (UTR) of the mouse gene. The chimeric exon contains nucleotides (nt) 1-117 of the mouse Prnp coding sequence, encoding amino acids (aa) 1-39, and nt 118-762 of the sheep PRNP coding sequence encoding aa 40-253 of the AHQ prion protein variant, which has alanine, histidine and glutamine at ovine polymorphic codons 136, 154 and 171 (A136H154Q171). This allele, while conferring relative resistance to infection of sheep with classic scrapie, is strongly associated with susceptibility to atypical scrapie. The transgene copy number of homozygous transgenic mice of line EM16 was estimated by Southern blot analysis to be 19 +/- 1. Expression of the chimeric protein in brain homogenates from homozygous transgenic mice of this line, estimated by western blot analysis using enhanced chemiluminescence and autoradiography for visualization and densitometric quantitation, is 8-16-fold that seen in normal ovine brainstem. (J:32214, J:223991)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:223991 Griffiths PC, et al., Overexpression of chimaeric murine/ovine PrP (A136H154Q171) in transgenic mice facilitates transmission and differentiation of ruminant prions. J Gen Virol. 2013 Nov;94(Pt 11):2577-86
• All: 2 reference(s)