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Endonuclease-mediated Allele Detail
Symbol: Dbhem2.1(flpo)Rray
Name: dopamine beta hydroxylase; endonuclease-mediated mutation 2.1, Russell Ray
MGI ID: MGI:5770774
Synonyms: Dbhem2.1(flpo)Rray, DBH p2a FlpO, DBH-p2a-FLPo, Dbhtm2(DBH-FLPo)Rray
Gene: Dbh  Location: Chr2:27055519-27073216 bp, + strand  Genetic Position: Chr2, 19.29 cM
Germline Transmission:  Earliest citation of germline transmission: J:251840
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Allele Type:    Endonuclease-mediated (Recombinase)
Mutation:    Insertion
Mutation detailsThe mouse line was generated by homologous recombination using the CRISPR/Cas9 technology. The coding sequences for a viral A2 peptide, followed by the coding sequence for optimized FLP recombinase, was inserted at the termination codon of the endogenous Dbh gene; a single lox2722 (modified LoxP) site marks the former position of a floxed neomycin resistance cassette that was deleted by Cre-mediated recombination. During translation of the bi-cistronic mRNA, the ribosome "skips" the peptide bond between the two C-terminal amino acids (glycine and proline) of the A2 peptide, resulting in production of functional mouse DBH enzyme joined to most of the short A2 peptide and, independently, flpo recombinase with an N-terminal proline. (J:94077, J:251840)
 Tissue activity of this recombinase allele
Driver: Dbh     Summary of all recombinase alleles driven by Dbh. Comparative matrix view of recombinase activities

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dbh Mutation:  10 strains or lines available
Original:  J:251840 Sun JJ, et al., Generation of Two Noradrenergic-Specific Dopamine-Beta-Hydroxylase-FLPo Knock-In Mice Using CRISPR/Cas9-Mediated Targeting in Embryonic Stem Cells. PLoS One. 2016;11(7):e0159474
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory