Col1a1^{tm5(tetO-Braf-rs1)Ppp}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm5(tetO-Braf-rs1)Ppp}
• Name: collagen, type I, alpha 1; targeted mutation 5, Pier Paolo Pandolfi
• MGI ID: MGI:5763655
• Synonyms: TRE-BPS^3UTR
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm5(tetO-Braf-rs1)Ppp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222864
• Parent Cell Line: C2 (Jaenisch) (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm5(tetO-Braf-rs1)Ppp} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Braf-rs1 (MGI:88191)

• Mutation details: Flp-mediated recombination inserted into the FRT site flanked docking site downstream of the gene a FRT-flanked cassette with polyadenylation signal sequence, tetracycline response element promoter, the full-length mouse Braf sequence, polyadenylation signal sequence and PGK promoter followed by a hygromycin resistance cassette. (J:222864)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

C2 (Jaenisch) ES cells are derived from ES cell line v6.5.

References

• Original: J:222864 Karreth FA, et al., The BRAF pseudogene functions as a competitive endogenous RNA and induces lymphoma in vivo. Cell. 2015 Apr 9;161(2):319-32
• All: 1 reference(s)