Ltbp1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ltbp1^{tm1c(EUCOMM)Wtsi}
• Name: latent transforming growth factor beta binding protein 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5763562
• Synonyms: Ltbp1^floxed
• Gene: Ltbp1 Location: Chr17:75312563-75699507 bp, + strand Genetic Position: Chr17, 46.24 cM, cytoband E3
• Alliance: Ltbp1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ltbp1 gene page

Mutation origin

• Mutant Cell Line: EPD0127_1_A03
• Germline Transmission: Earliest citation of germline transmission: J:224402
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 75555406 of Chromosome 17 upstream of the critical exon 8 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 8 at position 75556260. The critical exon 8 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:224402)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ltbp1 Mutation: 115 strains or lines available

References

• Original: J:224402 Horiguchi M, et al., Abrogation of both short and long forms of latent transforming growth factor-beta binding protein-1 causes defective cardiovascular development and is perinatally lethal. Matrix Biol. 2015 Apr;43:61-70
• All: 1 reference(s)