Chrna7^tm3.1Swr
Targeted Allele Detail

Summary

• Symbol: Chrna7^tm3.1Swr
• Name: cholinergic receptor, nicotinic, alpha polypeptide 7; targeted mutation 3.1, Scott W Rogers
• MGI ID: MGI:5763561
• Synonyms: alpha7^E260A, E260A Chrna7-IRES-tauGFP mice
• Gene: Chrna7 Location: Chr7:62748440-62862274 bp, - strand Genetic Position: Chr7, 34.47 cM
• Alliance: Chrna7^tm3.1Swr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229180
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 7 was modified to contain two nucleotide changes (CT to GG) that result in the amino acid substitution of alanine for glutamic acid at position 260 (E260A). A C-terminal hemagglutinin (HA) epitope tag (YPYDVPDY) was inserted. The IRES, MAPT (tau) and GFP reporter fusion was inserted into the 3' UTR. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the reporter fusion. (J:229180)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chrna7 Mutation: 49 strains or lines available

References

• Original: J:229180 Enioutina EY, et al., The nicotinic receptor Alpha7 impacts the mouse lung response to LPS through multiple mechanisms. PLoS One. 2015;10(3):e0121128
• All: 5 reference(s)