Gabra1tm1.1Krmr
Targeted Allele Detail
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Symbol: |
Gabra1tm1.1Krmr |
Name: |
gamma-aminobutyric acid type A receptor subunit alpha 1; targeted mutation 1.1, Richard Kramer |
MGI ID: |
MGI:5763100 |
Synonyms: |
alpha1-GABAsubA/subPhoRM, alpha1T125C, PhoRM |
Gene: |
Gabra1 Location: Chr11:42021766-42073757 bp, - strand Genetic Position: Chr11, 24.97 cM
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Alliance: |
Gabra1tm1.1Krmr page
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Germline Transmission: |
Earliest citation of germline transmission:
J:230569
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
129
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Allele Type: |
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Targeted (Not Applicable) |
Mutations: |
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Insertion, Single point mutation
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Mutation details: The allele was designed to introduce a threonine-to-cysteine mutation at codon 152 (T152C counting from the start codon; or T125C in the mature peptide sequence) into exon 5 of the gene. The targeting vector also includes a loxP-flanked neomycin selection cassette upstream of exon 5, as well as a C-to-T silent mutation in the exon 5 DNA sequence (to create a HindIII site upstream of T152C for genotyping). Cre-mediated recombination removed the floxed neo cassette.
(J:230569)
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Original: |
J:230569 Lin WC, et al., A Comprehensive Optogenetic Pharmacology Toolkit for In Vivo Control of GABA(A) Receptors and Synaptic Inhibition. Neuron. 2015 Dec 2;88(5):879-91 |
All: |
3 reference(s) |
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