Hras^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: Hras^tm1.1Ics
• Name: Hras proto-oncogene, GTPase; targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:5763073
• Synonyms: HRAS p.G12S
• Gene: Hras Location: Chr7:140770839-140773938 bp, - strand Genetic Position: Chr7, 86.48 cM
• Alliance: Hras^tm1.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:325021
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: ES cells containing Hras^tm1Xbr were retargeted to introduce a G to A and an A to C substitutions at the first and last bases of codon 12 of exon 1. This results in an amino acid substitution of serine for glycine. Cre-mediated recombination was used to remove the hygro STOP cassette. The final allele contains the G12S mutation and the IRES-beta-geo cassette. (J:325021)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hras Mutation: 29 strains or lines available

References

• Original: J:325021 Dard L, et al., HRAS germline mutations impair LKB1/AMPK signaling and mitochondrial homeostasis in Costello syndrome models. J Clin Invest. 2022 Apr 15;132(8):e131053
• All: 2 reference(s)