H3f3a^tm1.2Mag
Targeted Allele Detail

Summary

• Symbol: H3f3a^tm1.2Mag
• Name: H3.3 histone A; targeted mutation 1.2, Terry Magnuson
• MGI ID: MGI:5760308
• Synonyms: H3f3a^fl
• Gene: H3f3a Location: Chr1:180630125-180641127 bp, - strand Genetic Position: Chr1, 84.46 cM, cytoband D2.3
• Alliance: H3f3a^tm1.2Mag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225909
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A gene trap targeting vector containing an FRT site, attP site, neomycin resistance cassette, attB site, EGFP, FRT site, and loxP flanked exon 2 was inserted into intron 1 via homologous recombination. FLP-mediated recombination removed the FRT flanked neomycin cassette and EGFP gene, leaving loxP flanked exon 2 (J:225909)

Expression

In Mice Carrying this Mutation:

57 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H3f3a Mutation: 29 strains or lines available

References

• Original: J:225909 Jang CW, et al., Histone H3.3 maintains genome integrity during mammalian development. Genes Dev. 2015 Jul 1;29(13):1377-92
• All: 1 reference(s)