Ptchd1^{tm1a(KOMP)IcsOrl}
Targeted Allele Detail

Summary

• Symbol: Ptchd1^{tm1a(KOMP)IcsOrl}
• Name: patched domain containing 1; targeted mutation 1a, Institut de Transgenose
• MGI ID: MGI:5755971
• Synonyms: Ptchd1^tm1a
• Gene: Ptchd1 Location: ChrX:154356451-154406810 bp, - strand Genetic Position: ChrX, 72.38 cM
• Alliance: Ptchd1^{tm1a(KOMP)IcsOrl} page
• IMPC: Ptchd1 gene page

Mutation origin

• Mutant Cell Line: G14BD10-161
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The modified vector was inserted at position 155587519 of Chromosome X upstream of exon 2. (Build GRCm38). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA sequence, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 155587393. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:204739)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptchd1 Mutation: 11 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 4 reference(s)