Ano6^em3(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ano6^em3(IMPC)Tcp
• Name: anoctamin 6; endonuclease-mediated mutation 3, The Centre for Phenogenomics
• MGI ID: MGI:5754582
• Gene: Ano6 Location: Chr15:95688724-95872632 bp, + strand Genetic Position: Chr15, 50.66 cM
• Alliance: Ano6^em3(IMPC)Tcp page
• IMPC: Ano6 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele produced from project TCPR0236 at The Centre for Phenogenomics by injecting Cas9 mRNA and one guide RNA with the spacer sequence GGATCGGGTCTGTCCCGTTG. This resulted in an indel comprised of a 3-bp deletion from Chr15:95948364 to 95948366 (GRCm38) and a 2-bp insertion of AC into exon 13 (ENSMUSE00000275040) (GRCm38). This mutation is predicted to cause a frameshift with amino acid changes after residue 493 and early truncation 10 amino acids later (p.N494Kfs*10). (J:165963)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ano6 Mutation: 81 strains or lines available

References

• Original: J:165963 Centre for Modeling Human Disease, Alleles produced for the NorCOMM project by the Centre for Modeling Human Disease (Cmhd), Institute of Biomaterials & Biomedical Engineering, University of Toronto. MGI Direct Data Submission. 2010
• All: 3 reference(s)