Tg(tetO-Gja1,-EGFP)L11Kwi
Transgene Detail

Summary

• Symbol: Tg(tetO-Gja1,-EGFP)L11Kwi
• Name: transgene insertion L11, Klaus Willecke
• MGI ID: MGI:5754390
• Synonyms: Cx43TetOeGFP
• Transgene: Tg(tetO-Gja1,-EGFP)L11Kwi Location: unknown
• Alliance: Tg(tetO-Gja1,-EGFP)L11Kwi page

Transgene origin

• Strain of Origin: C57BL/6 or FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-Gja1,-EGFP)L11Kwi expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Gja1

• Mutation details: The transgene contains the tetracycline response element, comprising seven copies of the tetracycline operator (tetO) sequence, flanked on each side by a cytomegalovirus (CMV) minimal promoter and the coding sequence for either gap junction protein, alpha 1 (Gja1, also called Cx43) or enhanced green fluorescent protein (EGFP), each followed by the beta-globin insulator sequence. Expression of the tetracycline transactivator from a second transgene result in the expression of both Gja1 and the EGFP reporter. (J:82809, J:130466)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

The transgene construct was microinjected into C57BL/6 x FVB/N fertilized oocytes. (J:82809)

References

• Original: J:130466 Roell W, et al., Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia. Nature. 2007 Dec 6;450(7171):819-24
• All: 2 reference(s)