Efna5^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Efna5^{tm1c(EUCOMM)Wtsi}
• Name: ephrin A5; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5752597
• Synonyms: ephrinA5^fl
• Gene: Efna5 Location: Chr17:62911179-63188312 bp, - strand Genetic Position: Chr17, 32.57 cM
• Alliance: Efna5^{tm1c(EUCOMM)Wtsi} page
• IMPC: Efna5 gene page

Mutation origin

• Mutant Cell Line: EPD0445_2_D06
• Germline Transmission: Earliest citation of germline transmission: J:223076
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 62957609 of Chromosome 17 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 62958614. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:223076)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Efna5 Mutation: 27 strains or lines available

References

• Original: J:223076 Suetterlin P, et al., Target-independent ephrina/EphA-mediated axon-axon repulsion as a novel element in retinocollicular mapping. Neuron. 2014 Nov 19;84(4):740-52
• All: 1 reference(s)