Mcam^tm1.2Asly
Targeted Allele Detail

Summary

• Symbol: Mcam^tm1.2Asly
• Name: melanoma cell adhesion molecule; targeted mutation 1.2, Aurelie S Leroyer
• MGI ID: MGI:5752588
• Gene: Mcam Location: Chr9:44045958-44054024 bp, + strand Genetic Position: Chr9, 24.68 cM
• Alliance: Mcam^tm1.2Asly page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222474
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 7. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 2 through 7. Western blot analysis confirmed the absence of protein expression in lung microvascular endothelial cells. (J:222474)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcam Mutation: 38 strains or lines available

References

• Original: J:222474 Jouve N, et al., CD146 mediates VEGF-induced melanoma cell extravasation through FAK activation. Int J Cancer. 2015 Jul 1;137(1):50-60
• All: 4 reference(s)