Igh^tm10.1Fwa
Targeted Allele Detail

Summary

• Symbol: Igh^tm10.1Fwa
• Name: immunoglobulin heavy chain complex; targeted mutation 10.1, Frederick W Alt
• MGI ID: MGI:5752253
• Synonyms: IGCR1/CBE2^-
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220021
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: In intergenic control region 1 (IGCR1) the second CCCTC-binding factor (CTCF)-binding element (CBE2) was replaced with scrambled sequence that does not bind CTCF. A floxed neo cassette was also inserted between the two CBEs and subsequently removed by cre mediated recombination. (J:220021)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 47 strains or lines available

References

• Original: J:220021 Lin SG, et al., CTCF-binding elements 1 and 2 in the Igh intergenic control region cooperatively regulate V(D)J recombination. Proc Natl Acad Sci U S A. 2015 Feb 10;112(6):1815-20
• All: 1 reference(s)