Gli2^{tm1(Gli2*)Aliu}
Targeted Allele Detail

Summary

• Symbol: Gli2^{tm1(Gli2*)Aliu}
• Name: GLI-Kruppel family member GLI2; targeted mutation 1, Aimin Liu
• MGI ID: MGI:5751722
• Synonyms: Gli2^deltaCLRKIn
• Gene: Gli2 Location: Chr1:118761862-118981349 bp, - strand Genetic Position: Chr1, 52.17 cM, cytoband E2-E4
• Alliance: Gli2^{tm1(Gli2*)Aliu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221426
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding region of Gli2deltaCLR, the 3' UTR of Gli2, three tandem repeats of SV40 polyA sequence, and a floxed neomycin resistance cassette replaced 111 bp of exon 2 (the first coding exon) via homologous recombination. The Gli2deltaCLR protein fails to localize to the cilium but retains intrinsic transcriptional activity and responds to Sufu inhibition. Expression of mutant mRNA and protein is increased about 2-fold and 12-fold, respectively in homozygous embryos compared to wild-type controls. (J:221426)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gli2 Mutation: 169 strains or lines available

References

• Original: J:221426 Liu J, et al., The loss of Hh responsiveness by a non-ciliary Gli2 variant. Development. 2015 May 1;142(9):1651-60
• All: 1 reference(s)