Igh^tm2.1Feil
Targeted Allele Detail

Summary

• Symbol: Igh^tm2.1Feil
• Name: immunoglobulin heavy chain complex; targeted mutation 2.1, Robert Feil
• MGI ID: MGI:5751554
• Synonyms: cHS4
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224306
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: Two CTCF binding sites from the chicken beta-globin gene (cHS4) was inserted between Igh-J and Emu. Cre-mediated recombination removed the floxed neomycin resistance cassette. (J:224306)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 41 strains or lines available

References

• Original: J:224306 Puget N, et al., Insertion of an imprinted insulator into the IgH locus reveals developmentally regulated, transcription-dependent control of V(D)J recombination. Mol Cell Biol. 2015 Feb;35(3):529-43
• All: 1 reference(s)