Lipc^{tm1.1(LIPC*)1Hdic}
Targeted Allele Detail

Summary

• Symbol: Lipc^{tm1.1(LIPC*)1Hdic}
• Name: lipase, hepatic; targeted mutation 1.1, Helen Dichek
• MGI ID: MGI:5750884
• Synonyms: ciHLki, ciHL transgene
• Gene: Lipc Location: Chr9:70705410-70859503 bp, - strand Genetic Position: Chr9, 39.57 cM
• Alliance: Lipc^{tm1.1(LIPC*)1Hdic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229079
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Single point mutation
• Lipc^{tm1.1(LIPC*)1Hdic} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  LIPC (3990)

  Lipc	(MGI:96216)

  with catalytically inactive S145G mutation

• Mutation details: Mice with this knock-in mutation express a catalytically inactive human hepatic lipase (LIPC) under control of the regulatory sequences of the endogenous mouse Lipc gene and the downstream hepatic control sequence from the human apolipoprotein E (APOE) gene. A human LIPC cDNA in which an A-to-G transition at nucleotide position 559 results in replacement of the catalytic serine at amino acid position 145 of the enzyme by glycine (S145G) was inserted in-frame into exon 2 of the mouse gene following the translation initiating ATG codon. A loxP-flanked neomycin selection cassette has been deleted by Cre recombinase-mediated recombination. Western blot analysis using a rabbit polyclonal anti-human LIPC antiserum confirmed the presence of immunoreactive human hepatic lipase in sera of mice bearing this allele. Analysis of postheparin plasma detected no hepatic lipase activity in homozygous mutant mice. (J:229079, J:229080)
• Inheritance: Recessive

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lipc Mutation: 37 strains or lines available

References

• Original: J:229079 Chen J, et al., In vivo structure-function studies of human hepatic lipase: the catalytic function rescues the lean phenotype of HL-deficient (hl-/-) mice. Physiol Rep. 2015 Apr;3(4)
• All: 2 reference(s)