Syk^tm1(Syk)Spwa
Targeted Allele Detail

Summary

• Symbol: Syk^tm1(Syk)Spwa
• Name: spleen tyrosine kinase; targeted mutation 1, Steve P Watson
• MGI ID: MGI:5750743
• Synonyms: Syk^R41Afl
• Gene: Syk Location: Chr13:52737209-52802828 bp, + strand Genetic Position: Chr13, 27.41 cM, cytoband B-C2
• Alliance: Syk^tm1(Syk)Spwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220779
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Syk^tm1(Syk)Spwa expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Syk (MGI:99515)

• Mutation details: A loxP site, splice acceptor site, wild-type mouse Syk cDNA, FRT flanked neo cassette, loxP site, splice acceptor site, and exon 2 with an R41A mutation were inserted via homologous recombination. In the absence of cre mediated recombination the wild-type gene is expressed. Following cre mediated recombination a mutant protein lacking binding to phosphotyrosine at the N-terminal SH2 is expressed. (J:220779)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syk Mutation: 41 strains or lines available

References

• Original: J:220779 Hughes CE, et al., The N-terminal SH2 domain of Syk is required for (hem)ITAM, but not integrin, signaling in mouse platelets. Blood. 2015 Jan 1;125(1):144-54
• All: 1 reference(s)