Tg(tetO-lacZ,-GFP/Gria2)1Rsp
Transgene Detail

Summary

• Symbol: Tg(tetO-lacZ,-GFP/Gria2)1Rsp
• Name: transgene insertion 1, Rolf Sprengel
• MGI ID: MGI:5708835
• Synonyms: Tg^OCN1, TgTetOGFP-GluA2/1, Tg(tetO-GFP/Gria2)1Rsp
• Transgene: Tg(tetO-lacZ,-GFP/Gria2)1Rsp Location: unknown
• Alliance: Tg(tetO-lacZ,-GFP/Gria2)1Rsp page

Transgene origin

• Strain of Origin: C57BL/6 or DBA

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-lacZ,-GFP/Gria2)1Rsp expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Gria1 (50592)

  Gria1

  (MGI:95808)

  GFP fusion protein

• Mutation details: This transgene contains a tetracycline responsive element, comprising seven tandem copies of the tetracycline operator (tetO), driving bidirectional expression of beta-galactosidase (from the lacZ gene) with a nuclear localization signal and of a green fluorescent protein/rat glutamate receptor, ionotropic, AMPA2 (alpha 2) (GFP/Gria2) fusion protein; the GFP coding sequence is preceded by the signal sequence of the Drosophila cuticle protein C3 and the Myc epitope tag. (J:76021, J:103689)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

"Mouse lines TgTetOGFP-GluA2/1 and TgTetOGFP-GluA2/6 are derived from two diffferent founders from the same pronucleus injection" (J:82809). The transgenic construct was microinjected into the pronuclei of oocytes obtained from either "DBA1/C57Bl/6 F1 hybrids" (J:103689) or (C57BL/6 x DBA/2)F1 mice (J:82809).

References

• Original: J:103689 Shimshek DR, et al., Enhanced odor discrimination and impaired olfactory memory by spatially controlled switch of AMPA receptors. PLoS Biol. 2005 Nov;3(11):e354
• All: 2 reference(s)