Rab11a^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rab11a^{tm1c(KOMP)Wtsi}
• Name: RAB11A, member RAS oncogene family; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5708486
• Synonyms: Rab11a^fl, Rab11a^flox
• Gene: Rab11a Location: Chr9:64622582-64645038 bp, - strand Genetic Position: Chr9, 34.87 cM, cytoband C
• Alliance: Rab11a^{tm1c(KOMP)Wtsi} page
• IMPC: Rab11a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218246
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 64633665 of Chromosome 9 upstream of exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exons 2and 3 at position 64636163. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:218246)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab11a Mutation: 16 strains or lines available

References

• Original: J:218246 Sobajima T, et al., Rab11a is required for apical protein localisation in the intestine. Biol Open. 2014;4(1):86-94
• All: 2 reference(s)