Ppp5c^tm1.1Ptwc
Targeted Allele Detail

Summary

• Symbol: Ppp5c^tm1.1Ptwc
• Name: protein phosphatase 5, catalytic subunit; targeted mutation 1.1, Patricia TW Cohen
• MGI ID: MGI:5708332
• Synonyms: Ppp5^D274A
• Gene: Ppp5c Location: Chr7:16738575-16761812 bp, - strand Genetic Position: Chr7, 9.15 cM
• Alliance: Ppp5c^tm1.1Ptwc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218791
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Single point mutation
• Mutation details: A GAC to GCT codon change was inserted in exon 7 and an FRT flanked neo cassette was inserted in intron 6 via homologous recombination. The condon change results in an Asp274Ala substitution. Flp mediated recombination removed the neo cassette. Immunoblot analysis detected the presence of the 58 kDa form but not the cleaved 50kDa form in liver lysates from homozygous mice. The mutant protein lacks protein phosphatase activity. (J:218791)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp5c Mutation: 47 strains or lines available

References

• Original: J:218791 Jacob W, et al., Decreased adipogenesis and adipose tissue in mice with inactivated protein phosphatase 5. Biochem J. 2015 Feb 15;466(1):163-76
• All: 1 reference(s)