Retsat^tm1Kpal
Targeted Allele Detail

Summary

• Symbol: Retsat^tm1Kpal
• Name: retinol saturase (all trans retinol 13,14 reductase); targeted mutation 1, Krzysztof Palczewski
• MGI ID: MGI:5708113
• Synonyms: RetSat^-
• Gene: Retsat Location: Chr6:72575585-72584471 bp, + strand Genetic Position: Chr6, 32.27 cM, cytoband C3
• Alliance: Retsat^tm1Kpal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222367
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced with a neomycin selection cassette. RT-PCR of RNA isolated from the liver of homozygous mice using primers directed against exon 1 and exon 4 shows that there was no detectable message expressed from this allele. Immunoblotting of liver lysates and immunohistochemistry of liver sections from homozygous mice indicates that the encoded protein is not detectable in homozygous mice. (J:222367)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Retsat Mutation: 21 strains or lines available

References

• Original: J:222367 Moise AR, et al., Increased adiposity in the retinol saturase-knockout mouse. FASEB J. 2010 Apr;24(4):1261-70
• All: 4 reference(s)