Dicer1^tm1.1Doca
Targeted Allele Detail

Summary

• Symbol: Dicer1^tm1.1Doca
• Name: dicer 1, ribonuclease type III; targeted mutation 1.1, Donal O'Carroll
• MGI ID: MGI:5707512
• Synonyms: Dcr^FH, FH-Dicer, FlagHa2-Dicer
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^tm1.1Doca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228491
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: This allele encodes the wild-type dicer protein with N-terminal Flag-HA-HA epitope tags; the DNA sequence encoding the tags was inserted immediatedly downstream of the translation initiating ATG. A single loxP site in intron 2 marks the site of Cre recombinase excision of a loxP-flanked neomycin resistance cassette. Immunofluorescence analysis of testis sections using anti-HA antibodies demonstrated high expression of the tagged protein in mitotic spermatogonia and the early meiotic stages, increasing to a maximum in early pachytene spermatocytes and subsequently diminishing but remaining detectable in later stages of spermiogenesis. (J:228491)

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 94 strains or lines available

References

• Original: J:228491 Comazzetto S, et al., Oligoasthenoteratozoospermia and infertility in mice deficient for miR-34b/c and miR-449 loci. PLoS Genet. 2014 Oct;10(10):e1004597
• All: 2 reference(s)