Gt(ROSA)26Sor^{tm1(tetO-tdTomato,-BRAF*)Mmok}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(tetO-tdTomato,-BRAF*)Mmok}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Markus Morkel
• MGI ID: MGI:5707503
• Synonyms: tdTomato-BRAF^V600K
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(tetO-tdTomato,-BRAF*)Mmok} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222531
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(tetO-tdTomato,-BRAF*)Mmok} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BRAF (673)

  Braf	(MGI:88190)

  The cDNA encodes a protein with the amino acid substitution of lysine for valine at position 600 (V600K)

• Mutation details: Recombination using Gt(ROSA)26Sor^tm2Bgh replaced the hygromycin resistance cassette is replaced with tetracycline response element driving inducible expression of a tdTomato reporter gene, 2A peptide sequence and a human BRAF cDNA with the point mutation that results in the amino acid substitution of lysine for valine at position 600 (V600K) followed by a polyadenylation sequence. (J:222531)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:222531 Riemer P, et al., Transgenic expression of oncogenic BRAF induces loss of stem cells in the mouse intestine, which is antagonized by beta-catenin activity. Oncogene. 2015 Jun 11;34(24):3164-75
• All: 1 reference(s)