Afap1^tm1.2Ycho
Targeted Allele Detail

Summary

• Symbol: Afap1^tm1.2Ycho
• Name: actin filament associated protein 1; targeted mutation 1.2, Youngjin Cho
• MGI ID: MGI:5707264
• Synonyms: Afap1^{deltaexon 5}
• Gene: Afap1 Location: Chr5:36050713-36161267 bp, + strand Genetic Position: Chr5, 19.02 cM, cytoband B1
• Alliance: Afap1^tm1.2Ycho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221250
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 was floxed. Flp-mediated recombination removed the FRT-flanked selection cassette inserted downstream of exon 5. Cre-mediated recombination removed exon 5. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:221250)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Afap1 Mutation: 59 strains or lines available

References

• Original: J:221250 Cunnick JM, et al., Actin filament-associated protein 1 is required for cSrc activity and secretory activation in the lactating mammary gland. Oncogene. 2015 May 14;34(20):2640-9
• All: 1 reference(s)