Net1^tm1Jafr
Targeted Allele Detail

Summary

• Symbol: Net1^tm1Jafr
• Name: neuroepithelial cell transforming gene 1; targeted mutation 1, Jeffrey A Frost
• MGI ID: MGI:5707251
• Gene: Net1 Location: Chr13:3932018-3968220 bp, - strand Genetic Position: Chr13, 2.17 cM, cytoband A1
• Alliance: Net1^tm1Jafr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218290
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The vector PRPGS00153_A_F11 containing the L1L2_Bact_P cassette was inserted at position 3889355 of Chromosome 13 upstream of the exon 4 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 10 at position 3885819. Exons 4-10 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:218290)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Net1 Mutation: 31 strains or lines available

References

• Original: J:218290 Zuo Y, et al., The RhoGEF Net1 is required for normal mammary gland development. Mol Endocrinol. 2014 Dec;28(12):1948-60
• All: 1 reference(s)