Mapk6^tm2Melo
Targeted Allele Detail

Summary

• Symbol: Mapk6^tm2Melo
• Name: mitogen-activated protein kinase 6; targeted mutation 2, Sylvain Meloche
• MGI ID: MGI:5707044
• Synonyms: Erk3^KA, Mapk6^KD
• Gene: Mapk6 Location: Chr9:75294064-75317303 bp, - strand Genetic Position: Chr9, 42.3 cM, cytoband E1
• Alliance: Mapk6^tm2Melo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224353
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Lysine codons 49 (AAG) and 50 (AAA) in exon 2 were changed to alanine (GCA) (p.49_50delKKinsAA) and a loxP site flanked neomycin resistance gene was inserted into intron 2. The mutations prevent ATP binding to the kinase and renders it kinase-dead. (J:224353, J:294106)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapk6 Mutation: 29 strains or lines available

References

• Original: J:224353 Marquis M, et al., The catalytic activity of the mitogen-activated protein kinase extracellular signal-regulated kinase 3 is required to sustain CD4+ CD8+ thymocyte survival. Mol Cell Biol. 2014 Sep 15;34(18):3374-87
• All: 2 reference(s)