Nrp1^tm1.1Chgu
Targeted Allele Detail

Summary

• Symbol: Nrp1^tm1.1Chgu
• Name: neuropilin 1; targeted mutation 1.1, Chenghua Gu
• MGI ID: MGI:5705771
• Synonyms: Nrp1^VEGF-
• Gene: Nrp1 Location: Chr8:129085085-129229844 bp, + strand Genetic Position: Chr8, 75.78 cM, cytoband E
• Alliance: Nrp1^tm1.1Chgu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218050
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A D320K mutation was introduced into exon 6 and an FRT flanked neo cassette was inserted in intron 6 via homologous recombination. Flp mediated recombination removed the neo cassette. The mutation in exon 6 disrupts binding to VEGF without disrupting AP-SEMA3A binding. Western blot analysis indicates protein expression level is similar to wild-type controls at E14.5 in the lung and in the adult heart, brain, lung and kidney. (J:218050)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nrp1 Mutation: 81 strains or lines available

References

• Original: J:218050 Gelfand MV, et al., Neuropilin-1 functions as a VEGFR2 co-receptor to guide developmental angiogenesis independent of ligand binding. Elife. 2014;3:e03720
• All: 1 reference(s)