Usp18^tm1.1Kpk
Targeted Allele Detail

Summary

• Symbol: Usp18^tm1.1Kpk
• Name: ubiquitin specific peptidase 18; targeted mutation 1.1, Klaus-Peter Knobeloch
• MGI ID: MGI:5705585
• Synonyms: USP18^C61A
• Gene: Usp18 Location: Chr6:121222865-121247876 bp, + strand Genetic Position: Chr6, 57.17 cM, cytoband F
• Alliance: Usp18^tm1.1Kpk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217664
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An FRT flanked selection cassette was inserted in intron 2 and the codon in exon 3 for Cys61 5'-TGT-3' was mutated to 5-GCT-3' encoding alanine via homologous recombination. Flp mediated recombination removed the selection cassette. The mutation in exon 3 disrupts the isopeptidase activity of the protein. However regulatory properties of the protein are intact. (J:217664)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Usp18 Mutation: 25 strains or lines available

References

• Original: J:217664 Ketscher L, et al., Selective inactivation of USP18 isopeptidase activity in vivo enhances ISG15 conjugation and viral resistance. Proc Natl Acad Sci U S A. 2015 Feb 3;112(5):1577-82
• All: 9 reference(s)