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Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn
Transgene Detail
Nomenclature
Symbol: Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn
Name: transgene insertion 3, Karoly Mirnics
MGI ID: MGI:5703933
Synonyms: Pvalb/Gad1
Transgene: Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6NTac
Transgene
description
Transgene Type:    Transgenic (Conditional ready, Knockdown, Reporter)
Mutation:    Insertion
  Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn involves 1 genes/genome features (Gad1) View all
 
Mutation detailsThe transgenic construct uses 129.7 kbp from the bacterial artificial chromosome (BAC) RP24-306A6 possessing the parvalbumin locus (Pvalb) as well as ~65 kbp of centromeric sequences (including the complete Ift27 locus) and ~50 kbp of telomeric sequences (including the complete Ncf4 locus). Using homologous recombination/BAC recombineering, the following was inserted in-frame with the start codon of the BAC-encoded Pvalb: the red fluorescent protein tdTomato coding sequence, a lox2272 site, a human -globin minigene (exon1-intron-exon2) with the intron containing a miRNA:Gad1 sequence, a second lox2272 site and an SV40 polyA signal. An FRT-flanked neomycin resistance cassette was also present, but removed during BAC recombineering in vitro. The miRNA:Gad1 sequence is a fused miR30,miRNA:Gad1 synthetic miRNA sequence directed against the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA (mp ID 283874), with unique flanking miR-30 sequences. Also in vitro, the sequences encompassing exons 2-4 of the Ift27 locus were deleted. No other loci on the BAC were altered. (J:227902)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:227902 Brown JA, et al., Inhibition of parvalbumin-expressing interneurons results in complex behavioral changes. Mol Psychiatry. 2015 Dec;20(12):1499-507
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory