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Transgene Detail
Symbol: Tg(Cnr1-EGFP/RNAi:Gad1)1Mirn
Name: transgene insertion 1, Karoly Mirnics
MGI ID: MGI:5703789
Synonyms: Cnr1-eGFP-miRNA:Gad1, Cnr1/Gad1
Transgene: Tg(Cnr1-EGFP/RNAi:Gad1)1Mirn  Location: unknown  
Strain of Origin:  C57BL/6NTac
Transgene Type:    Transgenic (Conditional ready, Knockdown, Reporter)
Mutation:    Insertion
  Tg(Cnr1-EGFP/RNAi:Gad1)1Mirn involves 1 genes/genome features (Gad1) View all
Mutation detailsThe transgenic construct uses 151.7 kbp from the bacterial artificial chromosome (BAC) RP24-370M5 possessing the cannabinoid receptor 1 locus (Cnr1) as well as ~86 kbp of centromeric sequences and ~41 kbp of telomeric sequences; no other confirmed genes are present in the flanking sequences. Using homologous recombination/BAC recombineering, the following was inserted in-frame with the start codon of the BAC-encoded Cnr1: eGFP, a lox2272 site, a human -globin minigene (exon1-intron-exon2) with the intron containing a miRNA:Gad1 sequence, a second lox2272 site and an SV40 polyA signal. An FRT-flanked neomycin resistance cassette was also present, but removed during BAC recombineering in vitro. The miRNA:Gad1 sequence is a fused miR30,miRNA:Gad1 synthetic miRNA sequence directed against the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA (mp ID 283874), with unique flanking miR-30 sequences. No other loci on the BAC were altered. (J:210380)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:210380 Brown JA, et al., The role of cannabinoid 1 receptor expressing interneurons in behavior. Neurobiol Dis. 2014 Mar;63:210-21
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory