Rho^{tm5.1(RHO*/GFP)Jhw}
Targeted Allele Detail

Summary

• Symbol: Rho^{tm5.1(RHO*/GFP)Jhw}
• Name: rhodopsin; targeted mutation 5.1, John H Wilson
• MGI ID: MGI:5702734
• Synonyms: Q344X-hRho-GFP
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^{tm5.1(RHO*/GFP)Jhw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223491
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Rho^{tm5.1(RHO*/GFP)Jhw} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RHO (6010)

  Rho	(MGI:97914)

• Mutation details: The endogenous gene was replaced with a floxed HPRT minigene and a human RHO gene fused to GFP via homologous recombination. The human RHO gene contains a C-to-T nonsense mutation resulting in a premature stop codon in place of glutamic acid codon 344 (p.Q344*). Cre-mediated recombination removed the minigene. The loxP site in the 5' UTR of the human gene reduces expression about 5 fold by interfering with mRNA translation. (J:223491)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 49 strains or lines available

References

• Original: J:223491 Sandoval IM, et al., Abrupt onset of mutations in a developmentally regulated gene during terminal differentiation of post-mitotic photoreceptor neurons in mice. PLoS One. 2014;9(9):e108135
• All: 4 reference(s)